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Discussion on Research Summary: Efficacy of Commercial ELISA tests
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Moderator:
DrO
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Posted on Thursday, Apr 17, 2008 - 8:55 am:
The short of it is the commercial ELISA test performed poorly with a sensitivity of 26% and a specificity of 96% in the samples analysed. Translated that is it only identified 1/4 of the infected but when it did ID them it was right 96% of the time.
DrO
Equine Vet J. 2008 Mar;40(2):182-3.
The efficacy of a commercial ELISA as an alternative to virus neutralisation test for the detection of antibodies to EAV.
Duthie S, Mills H, Burr P.
Biobest Laboratories Ltd, 6 Charles Darwin House, The Edinburgh Technopole, Nr Penicuik, EH26 0PY, UK.
Infection with equine arteritis virus is a notifiable disease with sporadic occurrence in the UK. As stallions may harbour the virus after infection, horses are screened for exposure by serological testing prior to breeding. The virus neutralisation test is considered the 'gold standard' serological screening test, but it is time-consuming and labour intensive; consequently there is a move towards more rapid screening methodology. In this study, a commercially available EVA antibody ELISA is assessed. The ELISA performed poorly with a sensitivity of 26% and a specificity of 96% in the samples analysed. It was concluded that this ELISA would be of little value for reducing sample turnaround time. The study emphasises the need for in-house validation of commercially available kits.
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Member:
ekaufman
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Posted on Friday, Apr 18, 2008 - 12:39 am:
Dr. O, do you happen to know what the diagnostic labs are using for their pre-vaccination titers? I assume they are using virus neutralization, since it takes quite a while to get results, and isn't cheap.
(FWIW, my vet accidentally titered one of my vaccinated mares this year, and the lab caught it. Thought they had a rare positive in CO, but no such "luck.").
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Moderator:
DrO
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Posted on Wednesday, May 7, 2008 - 10:00 am:
Sorry for the slow respose Elizabeth but as far as I know VN is the standard.
DrO
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